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Using genome-scale fashions to optimize nutrient current for sustained algal progress and lipid productiveness
Nutrient availability is essential for progress of algae and completely completely different microbes used for producing worthwhile biochemical merchandise. Figuring out the optimum ranges of nutrient gives to cultures can get rid of feeding of extra dietary nutritional vitamins, reducing manufacturing prices and decreasing nutrient air air air pollution into the setting.
With the seems of omics and bioinformatics strategies, it’s now attainable to assemble genome-scale fashions that precisely describe the metabolism of microorganisms. On this research, a genome-scale mannequin of the inexperienced alga Chlorella vulgaris (iCZ946) was utilized to foretell feeding of lots of dietary nutritional vitamins, together with nitrate and glucose, beneath each autotrophic and heterotrophic circumstances.
The objective perform was modified from optimizing progress to as a substitute minimizing nitrate and glucose uptake costs, enabling predictions of feed costs for these dietary nutritional vitamins. The metabolic mannequin administration (MMC) algorithm was validated for autotrophic progress, saving 18% nitrate whereas sustaining algal progress.
Moreover, we obtained comparable progress profiles by concurrently controlling glucose and nitrate gives beneath heterotrophic circumstances for each excessive and low ranges of glucose and nitrate. Lastly, the nitrate current was managed in an effort to retain protein and chlorophyll synthesis, albeit at a decrease cost, beneath nitrogen-limiting circumstances.
This model-driven cultivation methodology doubled all of the volumetric yield of biomass, elevated fatty acid methyl ester (FAME) yield by 61%, and enhanced lutein yield nearly Three fold in contrast with nitrogen hunger. This research introduces a administration methodology that integrates omics information and genome-scale fashions in an effort to optimize nutrient gives based completely on the metabolic state of algal cells in a lot of nutrient environments.
This system may rework bioprocessing administration correct proper right into a methods biology-based paradigm relevant for numerous species in an effort to restrict nutrient inputs, within the discount of processing prices, and optimize biomanufacturing for the following experience of fascinating biotechnology merchandise.
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Should the Human Pim-2 Oncogene (PIM2) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Pim-2 Oncogene (PIM2) in samples from serum, plasma, tissue homogenates or other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
Known also as Pim-2 Oncogene Clia kit. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-2
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2)Serum, plasma, tissue homogenates and other biological fluids
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids.
Known also as Pim-2 Oncogene elisa. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-2
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-2 Oncogene (PIM2) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-2 Oncogene (PIM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-2 Oncoge
Description: A sandwich ELISA kit for detection of Pim-2 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Immunogen information: Synthesized peptide derived from the C-terminal region of human Pim-2
Applications tips:
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2
Immunogen information: Synthesized peptide derived from the C-terminal region of human Pim-2
Applications tips:
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2
Immunogen information: Synthesized peptide derived from the C-terminal region of human Pim-2
Applications tips:
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.
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CRISPR genome modifying has revolutionized genetics in quite a few organisms. Contained in the nematode Caenorhabditiselegans one injection into every of the 2 gonad arms of an grownup hermaphrodite exposes tons of of meiotic germ cells to modifying mixtures, allowing the restoration of lots of indels or small precision edits from every successfully injected animal. Sadly, significantly for extended insertions, modifying efficiencies can differ broadly, necessitating lots of injections, and usually requiring co-selection methods.
Correct proper right here we present that melting double stranded DNA (dsDNA) donor molecules earlier to injection will enhance the frequency of tangible homology-directed restore (HDR) by lots of fold for longer edits. We describe troubleshooting methods that allow persistently excessive modifying efficiencies ensuing, as an illustration, in as quite a bit as 100 unbiased GFP knock-ins from a single injected animal. These efficiencies make C. elegans by far the perfect metazoan to genome edit, eradicating limitations to the use and adoption of this facile system as a mannequin for understanding animal biology.
Longitudinal Assertion of Muscle Mass over 10 Years In step with Serum Calcium Ranges and Calcium Consumption amongst Korean Adults Aged 50 and Older: The Korean Genome and Epidemiology Evaluation
The intention of this research was to evaluation the longitudinal change in muscle mass over 10 years in step with serum calcium ranges and calcium consumption. A whole of 1497 males and 1845 ladies aged 50 years and older had been included. Crucial muscle loss (SML) was outlined as a 5% or larger loss from baseline, whereas time-dependent progress of SML was assessed in step with quartiles for corrected calcium stage and day by day calcium consumption utilizing Cox regression fashions.
The incidence of SML was 6.7 and seven.7 per 100-person-years amongst women and men, respectively. Teams with the underside corrected calcium ranges had extra wonderful SML than these with elevated calcium ranges, regardless of intercourse. The connection between SML and calcium consumption was crucial solely amongst ladies. The hazard ratio for SML per 1 mmol/L enhance in corrected calcium stage was 0.236 and 0.237 for women and men, respectively. In conclusion, low serum calcium ranges might predict SML amongst adults aged ≥ 50 years, whereas low calcium consumption can also be a predictor for muscle loss amongst ladies. Subsequently, encouraging dietary calcium consumption amongst middle-aged and older adults for preservation of muscle mass should be thought-about.
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Should the Human Pim-3 Oncogene (PIM3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Pim-3 Oncogene (PIM3) in samples from serum, plasma, tissue homogenates or other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.
Known also as Pim-3 Oncogene elisa. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-3
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-3 Oncogene (PIM3) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.
Known also as Pim-3 Oncogene Clia kit. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-3
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3)tissue homogenates and other biological fluids
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-3 Oncoge
Description: A sandwich ELISA kit for detection of Pim-3 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.
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